Methods for detecting plasticizers

摘要

Nanoparticles having one or more attached sensing moieties including uridine 5′-triphosphate (UTP) and deoxythymidine 5′-triphosphate (dTTP), are disclosed herein. These nanoparticles can, for example, be used for detection of plasticizers, such as phthalates, in the sample. Methods, kits and apparatuses using these nanoparticles for detecting plasticizers in a sample are also disclosed herein.

主权项

1. A method for detecting a phthalate in a sample, the method comprising:
providing a sample suspected of containing a phthalate; providing a plurality of nanoparticles having one or more attached sensing moieties, wherein the sensing moiety comprises one or more uridine 5′-triphosphate (UTP) groups, uridine 5′-diphosphate (UDP) groups, uridine monophosphate (UMP) groups, 2′-deoxythymidine 5′-triphosphate (dTTP) groups, 2′-deoxythymidine 5′-diphosphate (dTDP) groups, or deoxythymidine 5′-monophosphate (dTMP) groups; contacting the sample with the plurality of nanoparticles in the presence of a crosslinker to form a mixture, wherein the crosslinker is a copper (II) salt; maintaining the mixture under conditions to allow the crosslinker to bind the nanoparticles and any plasticizer present in the sample to form a nanoparticle aggregate; and detecting the nanoparticle aggregate, wherein the presence of the nanoparticle aggregate is indicative of the presence of a phthalate in the sample.