| 摘要 |
610129 Disclosed is a method for sequencing a nucleic acid sample, the method comprising: a. providing a plurality of particles, wherein each particle of said plurality is coupled to on average at most one nucleic acid molecule generated from said nucleic acid sample; b. performing nucleic acid amplification reactions on said nucleic acid molecules coupled to said particles, thereby producing isolated, clonally-amplified nucleic acid molecules coupled to at least a subset of said plurality of particles; c. performing a nucleic acid extension reaction using primers that hybridize to said clonally-amplified nucleic acid molecules; and d. detecting a local impedance change of each of said particles using a sensor array comprising a plurality of sensors to determine a sequence of each of said clonally-amplified nucleic acid molecules, thereby sequencing said nucleic acid sample. |
