PREPARATION OF FIBRIN GEL FOR USE AS IMPLANT SYSTEM

摘要

The present invention aims to generate a fibrin gel for cell proliferation and transport using the patient's own blood or compatible blood. The gel-preparation process consists in taking a quantity of blood from the patient, using sodium citrate as anticoagulant, separating off the citrated plasma by centrifugation, and resuspending the cells to be transported in the plasma. CaCl2 is added to the resulting suspension to form the gel (either as an isolated coagulate or within a porous chitosan, gelatin or hyaluronic acid matrix), and the gel is cultured in an incubator to promote cell growth. The invention will be of use in cell growth for the purposes of implantation in cutaneous, cartilaginous, gingival and bone lesions, inter alias, and in the field of tissue engineering.

主权项

1. A process for the preparation of an autologous fibrin gel or from compatible blood for cell vehiculization and proliferation, CHARACTERIZED in that comprises the steps of:
a) Taking an amount of blood from the own patient, at a rate of 200 DL per cm of implant to be prepared, using as anticoagulant sodium citrate at a concentration of 0.09 g per ml of blood; b) Separating the citrated plasma by centrifugation at 3000 rpm for ten minutes at 4° C. Re-suspending cells to be vehiculized in the plasma obtained in the above step; c) Adding to the suspension obtained in the previous step a solution of CaCl2 at 10% for forming the gel, either as an isolated clot or within a porous matrix. Culturing the gel in an incubator at 37° C. in humidified atmosphere and at 5% of CO2, for cell growth purposes.