| 发明名称 |
DETECTION OF MEMBRANE PROTEIN-THERAPEUTIC AGENT COMPLEXES BY MASS SPECTROMETRY |
| 摘要 |
According to the present invention, there is provided a method of detecting a complex comprising a membrane protein bound to a therapeutic agent by mass spectrometry. The method comprises: (a) providing a solution comprising a detergent micelle in which said complex is contained; (b) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (c) vaporising the solution using the nanoelectrospray ionisation source under conditions such that the complex is released from the micelle; (d) ionising the complex; (e) resolving the ionised complex using the mass analyser; and (f) detecting the resolved complex using the detector. Also provided is a solution comprising a detergent micelle in which a complex is contained, wherein the complex comprises a membrane protein bound to a therapeutic agent. |
| 申请公布号 |
US2014239169(A1) |
申请公布日期 |
2014.08.28 |
| 申请号 |
US201214126650 |
申请日期 |
2012.06.18 |
| 申请人 |
ISIS INNOVATION LIMITED |
发明人 |
Robinson Carol V.;Wang Sheila;Barrera Nelson P. |
| 分类号 |
H01J49/00;H01J49/02 |
主分类号 |
H01J49/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of detecting a complex comprising a membrane protein bound to a therapeutic agent by mass spectrometry, wherein the method comprises:
(a) providing a solution comprising a detergent micelle in which said complex is contained; (b) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (c) vaporising the solution using the nanoelectrospray ionisation source under conditions such that the complex is released from the micelle; (d) ionising the complex; (e) resolving the ionised complex using the mass analyser; and (f) detecting the resolved complex using the detector. |
| 地址 |
Oxford, Oxfordshire GB |
