| 发明名称 |
REGULATABLE PROMOTER |
| 摘要 |
A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system. |
| 申请公布号 |
US2014242636(A1) |
申请公布日期 |
2014.08.28 |
| 申请号 |
US201214350352 |
申请日期 |
2012.10.05 |
| 申请人 |
LONZA LTD. |
发明人 |
Mattanovich Diethard;Gasser Brigitte;Maurer Michael;Prielhofer Roland;Klein Joachim;Wenger Jana |
| 分类号 |
C12N15/63;C07K16/00;C12P21/00 |
主分类号 |
C12N15/63 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps
a) cultivating the cell line with a basal carbon source repressing the promoter, wherein the basal carbon source is a carbon source suitable for cell growth, b) cultivating the cell line with no or a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 20% as compared to the native pGAP promoter of the cell, and c) producing and recovering the POI. |
| 地址 |
Visp CH |
