SIMULTANEOUS DETECTION OF MULTIPLE MIRNAS USING CAPILLARY ELECTROPHORESIS SYSTEM EQUIPPED WITH PLURAL LASER-INDUCED FLUORESCENCE DETECTORS

摘要

The present invention relates to a method for simultaneously detecting multiple miRNAs present in a sample and a kit for detecting same. According to the present invention, two or more miRNAs existing in trace amounts in a sample can be analyzed through only one measurement. The detection method of the present invention may be used for fast diagnosis of various diseases wherein miRNAs are involved, for example, cardiovascular diseases including myocardial infarction, with high accuracy.

主权项

1. A method for simultaneously detecting two or more miRNAs present in a sample, comprising:
extracting RNAs from a sample to be analyzed; hybridizing the extracted RNAs with single-stranded DNAs labeled with fluorescent materials as probes specific for two or more miRNAs expected to exist in the sample in a hybridization buffer; identifying DNA-miRNA complexes using a capillary electrophoresis system equipped with a plurality of laser-induced fluorescence (LIF) detectors; and detecting the two or more miRNAs existing in the sample by detecting peaks of the DNA-miRNA complexes at different wavelengths.