METHOD FOR THE ENRICHMENT OF REBAUDIOSIDE B AND/OR REBAUDIOSIDE D IN STEVIA-DERIVED GLYCOSIDE COMPOSITIONS USING ADSORB-DESORB CHROMATOGRAPHY WITH A MACROPOROUS NEUTRAL ADSORBENT RESIN

摘要

The invention relates to the use of adsorb/desorb chromatography to prepare enriched compositions comprising rebaudioside B and/or rebaudioside D. Compositions with enriched rebaudioside-B and/or rebaudioside-D components may be prepared from Stevia-derived glycoside compositions using an adsorb-desorb chromatography process where the stationary phase of the chromatography bed comprises a macroporous neutral adsorbent resin.

主权项

1. A method of preparing an enriched composition comprising at least one of rebaudioside B, rebaudioside D, or a mixture thereof, the method comprising the steps of:
(A) providing a pH adjusted Stevia-derived glycoside solution comprising: (i) at least one glycoside selected from the group of reb-B and reb-D, and (ii) at least one other Stevia-derived glycoside; (B) providing a macroporous neutral adsorbent resin; (C) contacting the macroporous neutral adsorbent resin with the Stevia-derived glycoside solution so that at least a portion of glycosides in the Stevia-derived glycoside solution are adsorbed onto the macroporous neutral adsorbent resin; (D) providing at least one elution solvent comprising an ethanol and water mixture formulated to selectively elute reb-B, reb-D, or a mixture thereof from the adsorbent; (E) contacting the macroporous neutral adsorbent resin with the ethanol and water elution solvent in order to elute a composition enriched with at least one glycoside selected from the group of reb-B and reb-D; and (F) optionally, eluting at least a portion of the remaining adsorbed glycosides from the macroporous neutral adsorbent resin.