发明名称 |
PAN-YEAST AUTONOMOUSLY REPLICATING SEQUENCE |
摘要 |
A DNA sequence that functions as an origin in many different yeast species. From 1 to 17 mutations can be introduced into this sequence to improve its function across multiple yeasts. The resulting synthetic DNA sequence confers stable plasmid replication function in all yeast species tested, including but not limited to Saccharomyces cerevisiae, Lachancea kluyveri, Kluyveromyces lactis, Kluyveromyces wickerhammii, Hansenula polymorpha, and Pichia pastoris. Also provided are sequences that function as an optimal origin in the industrially useful Pichia pastoris. |
申请公布号 |
WO2014131056(A1) |
申请公布日期 |
2014.08.28 |
申请号 |
WO2014US18446 |
申请日期 |
2014.02.25 |
申请人 |
UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION |
发明人 |
DUNHAM, MAITREYA;LIACHKO, IVAN |
分类号 |
C12N15/113;C12N1/19;C12N15/63;C12N15/67;C12N15/81 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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地址 |
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