METHOD FOR USING DIRECTING CELLS FOR SPECIFIC STEM/PROGENITOR CELL ACTIVATION AND DIFFERENTIATION

摘要

A method is provided, including obtaining a population of antigen-presenting cells, enriching a population of stem/progenitor cells within a larger population of cells, activating the population of antigen-presenting cells and, following the activating, inducing at least one process selected from the group consisting of: differentiation, expansion, activation, secretion of a molecule, and expression of a marker, by exposing the enriched stem/progenitor cell population to the population of antigen-presenting cells. Other applications are also described.

主权项

1. A method for generating a lineage specific precursor/progenitor cell population (LSP), comprising:
(a) obtaining a population of cells comprising isolated immature antigen-presenting cells; (b) activating the population of cells comprising the isolated immature antigen-presenting cells to generate a population of cells comprising activated antigen-presenting cells; (c) obtaining a population of cells comprising stem/progenitor cells and enriching for the stem/progenitor cells within the population of cells to generate a population of cells comprising an enriched population of stem/progenitor cells; and (d) following the activating, combining (1) a population of cells selected from the group consisting of:
(i) the population of cells comprising the activated antigen-presenting cells; and(ii) a mixed population of cells comprising the population of cells comprising the activated antigen-presenting cells and a population of cells comprising effector cells, wherein the effector cells of the mixed population of cells are activated by mixing the effector cells with the population of cells comprising the activated antigen-presenting cells in forming the mixed population of cells, wherein responsive to the mixing, the activated antigen-presenting cells direct the activating of the effector cells; with (2) the population of cells comprising the enriched population of the stem/progenitor cells of step (c) to form a combined population of cells, wherein the population of cells of step (d)(i) or the mixed population of cells of step (d)(ii) directs at least the enriched population of the stem/progenitor cells of the combined population to induce at least one process selected from the group consisting of: differentiation, activation, secretion of a molecule, and expression of a marker, thereby generating a lineage specific precursor/progenitor population (LSP) from the enriched population of the stem/progenitor cells.