PATHOGEN DIAGNOSIS SYSTEM

摘要

The present invention is a pathogen diagnosis system for identifying pathogen(s) involved in developing infection wherein there are a plural of pathogens which may be involved, comprising: a body fluid collecting means for collecting part of body fluid being provided from a test body and containing DNA; a DNA extraction reagent for extracting DNA from the collected body fluid by said body fluid collecting means to obtain DNA extract; a PCR reagent for amplification reaction of DNA on said DNA extract, comprising thermostable DNA polymerase, buffer, deoxynucleoside triphosphates, and labeling agent being spectroscopically detectable by reacting with the amplified DNA; a plural types of primer reagents each comprising one or more kinds of primers for amplification of a target DNA having sequence distinguishing each of a plural of pathogens, each primer corresponding to each of said pathogens; a DNA extraction container for providing a field for DNA extraction reaction by use of said DNA extraction reagent; an extract collecting means for collecting part of DNA extract obtained in said DNA extraction container; a substrate containing at least the same number of micro flow channels as the number of pathogens or of groups of pathogens to be detected, in said micro flow channels said DNA extract collected by said extract collecting means, said PCR reagent and said primer reagent being loaded, and a field for DNA amplification reaction being provided; and a thermal cycler for controlling the temperature for DNA amplification reaction in said micro flow channel by heating or cooling said substrate, wherein prior to the DNA amplification reaction primer(s) is allowed to be introduced in each of said micro flow channels, and wherein one primer is not introduced in two or more micro flow channels.