METHOD FOR DETECTING THE INSERTION OF SPACERS IN CRISPR STRUCTURES

摘要

The invention relates to a method for detecting the insertion of spacers by means of independent selection of the interference from artificial structures based on clustered regularly interspaced short palindromic repeats (CRISPR) comprising the insertion of at least one CRISPR-spacer unit into the artificial structure in the sequence which encodes a reporter gene which restores the translation reading model, where the expression of the reporter gene is indicative of the insertion of the spacer. The invention also relates to the artificial structure used.