发明名称 |
Electrospray and nanospray ionization of discrete samples in droplet format |
摘要 |
<p>Droplets or plugs within multiphase microfluidic systems have rapidly gained interest as a way to manipulate samples and chemical reactions on the femtoliter to microliter scale. Chemical analysis of the plugs remains a challenge. It has been discovered that nanoliter plugs of sample separated by air or oil can be analyzed by electrospray ionization mass spectrometry when pumped directly into a fused silica nanospray emitter nozzle. Using leu-enkephalin in methanol and 1% acetic acid in water (50:50 v:v) as a model sample, we found carry-over between plugs was < 0.1% and relative standard deviation of signal for a series of plugs was 3%. Detection limits were 1 nM. Sample analysis rates of 0.8 Hz were achieved by pumping 13 nL samples separated by 3 mm long air gaps in a 75 ?m inner diameter tube. Analysis rates were limited by the scan time of the ion trap mass spectrometer. The system provides a robust, rapid, and information-rich method for chemical analysis of sample in segmented flow systems.</p> |
申请公布号 |
AU2010262978(B2) |
申请公布日期 |
2014.08.28 |
申请号 |
AU20100262978 |
申请日期 |
2010.06.18 |
申请人 |
MILESTONE DEVELOPMENT SERVICES;THE REGENTS OF THE UNIVERSITY OF MICHIGAN;NEW OBJECTIVE. INC. |
发明人 |
KENNEDY, ROBERT;PEI, JIAN;LI, QIANG;LEE, MIKE S.;VALASKOVIC, GARY A. |
分类号 |
G01N1/28 |
主分类号 |
G01N1/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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