ANALYTICAL METHOD AND DEVICE FOR MALIC ACID

摘要

Analytical methods and devices according to the present disclosure may be used to assay the concentration of malic acid, or related species, in a sample, e.g., preparations of fruit, vegetables, juice, and/or wine. Liquid samples are combined with a reaction mixture, incubated, and a parameter characteristic of the resulting incubated mixture is measured. During the incubation, the reaction mixture generally reacts with the liquid sample to transform malic acid or related species that is present in the liquid sample. The measured parameter may be pressure, pH, an amount (e.g., volume, mass) of CO2 generated during the incubation, a parameter related to the foregoing, and/or any other parameter related to the amount of malic acid or related species transformed.

主权项

1. A method of detecting malic acid, or related species, in a liquid sample comprising:
combining a liquid sample and a reaction mixture to create a combined mixture; incubating the combined mixture to create an incubated mixture; and measuring a measured parameter characteristic of the incubated mixture; wherein the reaction mixture includes a source of enzyme to transform malic acid or related species and wherein an activity concentration of the enzyme in the combined mixture is greater than 0.1 U/mL.