Heat-Transfer Resistance Based Analysis of Bioparticles

摘要

A bio-sensing device suitable for the detection and/or characterization of target bioparticles and corresponding method is described. The bio-sensing technique is based on the impact on the heat transfer resistivity value of bioparticles binding in binding cavities of a structured substrate. By sensing temperatures and determining a heat transfer resistivity value based thereon, a characteristic of the target bioparticles can be derived.

主权项

1. A bio-sensing device suitable for the detection and/or characterization of target bioparticles, the bio-sensing device comprising:
a heating element for heating using a power; a sample holder comprising a structured substrate having a surface comprising a plurality of binding cavities in which target bioparticles can bind, the sample holder further being adapted for exposing the structured substrate at one side to the heating element; a first temperature sensing element for sensing a temperature at the side where the structured substrate can be exposed to the heating element and a second temperature sensing element for sensing a temperature at the side opposite thereto with respect to the structured substrate; a processing means programmed for calculating at least one heat transfer resistivity value based on temperature values obtained with the first temperature sensing element and the second temperature sensing element and the power for the heating element, for deriving a characteristic of the target bioparticles from said heat transfer resistivity value.