发明名称 |
METHOD FOR DETERMINING FUNCTIONAL ACTIVITY OF IONISED CALCIUM IN HUMAN BLOOD SERUM |
摘要 |
FIELD: medicine.SUBSTANCE: invention can be used for determining functional activity of ionised calcium (FAI Ca) in human blood serum. The method for determining FAI Cais based on conducting a bovine erythrocyte lysis reaction in 10% human blood serum at temperature 37°C for 10 min in the presence of 0.55 mM ethylene glycol tetraacetic acid in a buffer for 10 min. An incubation is followed by determining a degree of inhibition of erythrocyte lysis; if observing a complement activity inhibition of less than 30%, a high functional activity of ionised calcium in a human body is stated; the inhibition of 31% to 70% shows the normal activity, and the inhibition of more than 71% - the low activity.EFFECT: using easy and fast to implement method enables detecting preclinical conditions of immune deficiency, can be used as a specific marker for an immunomodulatory therapy, provides conducting the in-depth study of autoimmune pathogenesis, ensuring early prevention thereof and controlling the effectiveness of the conducted therapy.5 ex, 6 tbl |
申请公布号 |
RU2526824(C1) |
申请公布日期 |
2014.08.27 |
申请号 |
RU20130139927 |
申请日期 |
2013.08.28 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUT NORMAL'NOJFIZIOLOGII IM. P.K. ANOKHINA" ROSSIJSKOJ AKADEMIIMEDITSINSKIKH NAUK (FGBU "NIINF IM. P.K. ANOKHINA" RAMN) |
发明人 |
SHOJBONOV BATOZHAB BATOZHARGALOVICH;GRIGOR'EVA DIANA VIKTOROVNA;KOSTYREVA MARINA VLADIMIROVNA;SHABALINA ALLA ANATOL'EVNA;ROMANOVA ELIZAVETA PETROVNA;TOLPYGO SVETLANA MIKHAJLOVNA;PEVTSOVA ELENA IGOREVNA;KOTOV ALEKSANDR VLADIMIROVICH |
分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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