RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli

摘要

FIELD: biotechnology.SUBSTANCE: invention is recombinant plasmid DNA encoding hybrid polypeptides with the properties of red fluorescent protein mCherry. The recombinant plasmid DNA pChFN3 encodes the hybrid protein ChFN3 which has the properties of red fluorescent protein mCherry and 10 domain of human fibronectin of type III, and the recombinant plasmid DNA pChTNF encodes the hybrid protein ChTNF which has the properties of red fluorescent protein mCherry and TNF. The present invention provides efficient production of hybrid proteins pChFN3 and ChTNF with the properties of red fluorescent protein mCherry in the strain E.coli BL21 (DE3).EFFECT: invention enables to obtain the target protein in high yield by adding a smaller amount of inducer.2 cl, 3 dwg, 8 ex