Immunity evaluation method, immunity evaluation apparatus, immunity evaluation program and data recording medium having the immunity evaluation program stored therein

摘要

An immunity evaluation method for evaluating immunity by using immune cell markers for immune cells contained in sampled blood comprises the step of determining an evaluation value for each of two or more selected kinds of immune cell markers based on the individual immune cell markers contained in the sampled blood, the step of adding the evaluation values so obtained for the at least two selected kinds of immune cell markers, and the step of evaluating the immunity from the results of the adding.

主权项

1. A method for evaluating the immunity status of a subject based on immune cells parameters in a blood sample from the subject, the method comprising the steps of:
(i) obtaining a blood sample from a subject and determining the values of at least two immune cell parameters in the blood sample, wherein said immune cell parameters include T cell count and T cell proliferation index, said T cell proliferation index being the product of T cell counts and T cell proliferation activity, wherein T cell proliferation activity is determined by collecting mononuclear cells by culturing them in the presence of a mitogenic agent, and determining the number of live cells in the sample of stimulated mononuclear cells using an assay, wherein T cell proliferation activity is measured; (ii) providing a database stored in a storage unit, the database comprising the values of said immune cell parameters of a plurality of healthy subjects, wherein the values of said immune cell parameters are ranked in a stepwise manner from low values to high values based on the cumulative frequency of said immune cell parameters in a plurality of healthy subjects, each step being accorded a specific numerical score; (iii) assigning a numerical score to each of the immune cell parameters of the subject by executing a scoring operation, wherein the value of each immune cell parameter determined in step (i) is compared to the values of the same immune cell parameters stored in the database provided in step (ii), the numerical score assigned to each immune cell parameter being related to the numerical score of the corresponding value of same parameter stored in the database; (iv) calculating the immunity score of the subject as the sum of the numerical scores assigned to each of the immune parameters in step (iii); (v) providing a database stored in a storage unit, the database comprising immunity scores of a plurality of healthy subjects, wherein the immunity scores are ranked in a stepwise manner from low immunity scores to high immunity scores based on the cumulative frequency of immunity scores in a plurality of healthy subjects, wherein ranges of immunity scores are identified as at least two level immunities from low immunity to high immunity, and (vi) comparing the immunity score of the subject calculated in step (iv) to immunity scores stored in the database provided in step (v) to thereby evaluate the immunity status of the subject as one of low immunity, normal immunity and high immunity.