Compositions and methods for immunostimulatory RNA oligonucleotides

摘要

The present invention provides 4-nucleotide (4mer) RNA motifs that confer immunostimulatory activity, in particular, IL-12-inducing activity to a single-stranded RNA oligonucleotide. The present invention also provides single-stranded RNA oligonucleotides, including antisense RNA, with high or low immunostimulatory activity. The present invention further provides the use of the RNA oligonucleotides of the invention for therapeutic purposes.

主权项

1. A method for preparing an antisense RNA oligonucleotide having gene silencing activity and high IL-12 inducing activity, comprising the steps of:
(a) identifying all potential antisense sequences between 18 and 50 nucleotides in length, for a target mRNA; (b) identifying antisense sequences that have gene silencing activity; (c) identifying antisense RNA sequences from those among identified in step (b) which have an IL-12 score of at least 8.4064×n+66.958, wherein the IL-12 score is assigned according to the method comprising the steps of:
(i) identifying all possible 3-nucleotide (3mer) motifs contained in the oligonucleotide;(ii) assigning an IL-12 point score for each individual 3mer motif
(A) for a 3mer motif which appears in Table 5, assign an IL-12 point score according to Table 5;(B) for a 3mer motif which does not appear in Table 5, assign an IL-12 point score of 0;(iii) assigning the sum of the IL-12 point scores of individual 3mer motifs as the IL-12 score of the oligonucleotide; and wherein n is the length of the sequence and n is between 18 and 50; (d) decreasing the IL-12 score threshold by 1 if no antisense sequence is identified in step (c), until at least one antisense sequence is identified; (e) preparing the antisense RNA identified in step (c) or (d); and (f) optionally testing the gene silencing and/or the IL-12 inducing activity of the antisense prepared in (e).