| 主权项 |
1. An analogue of human insulin where the analogue contains one additional disulfide bond not present in human insulin and wherein
i) the three disulfide bonds of human insulin are retained, ii) one amino acid selected from the group consisting of positions A9, A10 and A12 of the A chain of human insulin is substituted with a cysteine, iii) one amino acid selected from the group consisting of positions B1, B2, B3, B4, B5 and B6 of the B chain of human insulin is substituted with a cysteine, iv) at least one amino acid selected from the group consisting of positions A14, A21, B1, B3, B10, B16, B22, B25, B26, B27, B28, B29 and B30 of human insulin is substituted with an amino acid that is not a cysteine wherein if the amino acid in iii) is a cysteine at position B1 or B3, the same amino acid at position B1 or B3 cannot be an amino acid which is not a cysteine, and v) optionally the amino acid at position B27 and/or B30 of human insulin is deleted. |
