Multiplanar lateral flow assay with diverting zone

摘要

A lateral flow device includes a sample compressor and a test strip comprising a diverting zone. The diverting zone, which may include a barrier and/or a gap or ditch, stops or impedes flow. Flow is reinitiated and diverted into an alternate plane by compression of a sample compressor. Flow returns to the original, lateral plane, at the end of the diverting zone.

主权项

1. A lateral flow device for detecting an analyte in a sample comprising:
a sample compressor; a test strip comprising a test zone and a diverting zone upstream of the test zone, wherein the diverting zone interrupts lateral flow on the test strip; a conjugate comprising a first binding partner for the analyte and a label; and a second binding partner for the analyte; and a sample application zone where sample is applied to the lateral flow device, wherein the sample application zone is located in a location selected from the group consisting of: i) on the test strip upstream of the detection zone; ii) on the sample compressor; and iii) on a sample collector comprising a sample collection portion for collection of the sample; wherein a component selected from the group consisting of the conjugate, the second binding partner and both the conjugate and the second binding partner is not located on the test strip prior to use of the lateral flow device; and wherein the sample compressor creates a bridge over the diverting zone to divert flow onto the sample compressor and return flow to the test strip at the end of the diverting zone.