发明名称 |
Use of DNAzymes for analysis of an RNA sample |
摘要 |
Provided herein is method comprising: contacting an initial RNA sample containing a population of different RNA molecules with a divalent cation and a set of DNAzymes that are designed to cleave multiple target RNAs in the initial sample, thereby producing a product RNA sample that comprises: a) uncleaved RNA molecules and b) cleaved RNA fragments that contain a 2′,3′-cyclic-phosphate and a 5′ hydroxyl as the result of DNAzyme cleavage. |
申请公布号 |
US8815782(B2) |
申请公布日期 |
2014.08.26 |
申请号 |
US201213666752 |
申请日期 |
2012.11.01 |
申请人 |
Agilent Technologies, Inc. |
发明人 |
Zeiner Gusti;Ach Robert A. |
分类号 |
C40B50/06 |
主分类号 |
C40B50/06 |
代理机构 |
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代理人 |
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主权项 |
1. A method comprising: contacting an initial RNA sample containing a population of different RNA molecules with a divalent cation and a set of DNAzymes that are designed to cleave multiple target RNAs in said initial sample, thereby producing a product RNA sample that comprises: a) uncleaved RNA molecules and b) cleaved RNA fragments that contain a 2′,3′-cyclic-phosphate and a 5′ hydroxyl as the result of DNAzyme cleavage; and
ligating a first adaptor onto the 3′ ends of at least some of said cleaved RNA fragments using a 2′,3′-cyclic phosphate-specific RNA ligase, thereby producing a ligated sample comprising ligation products. |
地址 |
Santa Clara CA US |