Thin-layer chromatography and colorimetric analysis of multi-component explosive mixtures

摘要

A thin-layer chromatography method for detection and identification of common military and peroxide explosives in samples includes the steps of provide a reverse-phase thin-layer chromatography plate; prepare the plate by marking spots on which to deposit the samples by touching the plate with a marker; spot one micro liter of a first standard onto one of the spots, spot one micro liter of a second standard onto another of the spots, and spot samples onto other of spots producing a spotted plate; add eluent to a developing chamber; add the spotted plate to the developing chamber; remove the spotted plate from the developing chamber producing a developed plate; place the developed plate in an ultraviolet light box; add a visualization agent to a dip tank; dip the developed plate in the dip tank and remove the developed plate quickly; and detect explosives by viewing said developed plate.

主权项

1. A thin-layer chromatography method using a single thin-layer chromatography plate and a single colorimetric reagent for detection and identification of both military explosives and peroxide explosives in samples using the single thin-layer chromatography plate and using the single colorimetric reagent, consisting of the steps of:
providing a single reverse-phase thin-layer chromatography plate for detection and identification of both military explosives and peroxide explosives in samples; preparing said single reverse-phase thin-layer chromatography plate for detection and identification of both military explosives and peroxide explosives in sample by marking spots on which to deposit the samples; spotting one micro liter of a first standard onto one of said spots wherein said first standard is a standard for military explosives, spotting one micro liter of a second standard onto another of said spots wherein said second standard is a standard for peroxide explosives, and spotting samples onto other of said spots producing a spotted single reverse-phase thin-layer chromatography plate for detection and identification of both military explosives and peroxide explosives in sample; adding eluent to a developing chamber; adding said spotted single reverse-phase thin-layer chromatography plate to said developing chamber; removing said spotted single reverse-phase thin-layer chromatography plate from said developing chamber producing a developed single reverse-phase thin-layer chromatography plate; placing said developed single reverse-phase thin-layer chromatography plate in an ultraviolet light box; adding a single colorimetric visualization reagent to a dip tank, wherein said single colorimetric visualization reagent is diphenylbenzidine in EtOH; dipping said developed single reverse-phase thin-layer chromatography plate in said dip tank and remove said developed single reverse-phase thin-layer chromatography plate; and detecting the military explosives and detecting the peroxide explosives by viewing said developed single reverse-phase thin-layer chromatography plate.