Methods of generating neural stem cells

摘要

The present disclosure provides methods of generating neural stem cells from differentiated somatic cells. The present disclosure also provides induced neural stem cells generated using a subject method, as well as differentiated cells generated from a subject induced neural stem cell. A subject neural stem cell, as well as differentiated cells derived from a subject neural stem cell, is useful in various applications, which are also provided in the present disclosure.

主权项

1. A method of generating a multipotent induced neural stem cell, the method comprising:
a) genetically modifying a somatic cell with a nucleic acid comprising a nucleotide sequence encoding an exogenous Sox2 polypeptide; and b) culturing the genetically modified somatic cell on a glass substrate that is coated with laminin and/or gelatin, wherein said culturing results in generation of a multipotent induced neural stem cell (iNSC).