Method for culturing mycoplasma contamination-free cells and method for removing mycoplasma contamination of cells

摘要

Provided is a method for culturing cells, which prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using a fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, provided is a method for removing mycoplasma contamination of the cells, including a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.

主权项

1. A cell culturing method for preventing mycoplasma contamination, the cell culturing method comprising culturing cells in a medium that comprises
(i) an extract of biomass obtained by culturing Amphidinium klebsii or Amphidinium carterae, wherein the extract is obtained by performing an extraction process which comprises: (a) extracting the biomass with a C1 to C4 alcohol and acetone; (b) extracting the extract obtained from the step (a) with water and ethyl acetate, followed by separating an aqueous layer; and (c) extracting the aqueous layer obtained from the step (b) with n-butanol, followed by separating an n-butanol layer; or (ii) an amphidinol derivative selected from the group consisting of the compounds represented by Formula 1 to 8 below: thereby the cell culturing method prevents mycoplasma contamination in the resultant cell culture.