Polyelectrolyte-Coated Ion-Exchange Particles

摘要

A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.

主权项

1. A particle for separating PCR reaction products comprising:
a core comprising ion-exchange material; and a coating covering the exterior surface of the particle wherein the coating comprises a linear polyelectrolyte polymer, wherein the polyelectrolyte polymer creates a size exclusion barrier allowing small molecules having a size less than 300 bp to penetrate into the particle and restricting large molecules from interacting with the core; and wherein the particle is produced by exposing the core to an excess of the polyelectrolyte polymer.