| 主权项 |
1. A method for introducing a hydroxy or a keto group at the second or third carbon of at least two ends of a substituted or unsubstituted, linear or branched, aliphatic hydrocarbon having at least five carbons and having at least one hydrogen attached to said second or third carbon, comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity; wherein the polypeptide comprises:
a) an amino acid sequence which has at least 30% identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28 or 29; and b) an amino acid sequence represented by one or more of the following motifs:(SEQ ID NO: 9)Motif I:[FL]XX[YF]S[AN]X[FHY]G[GN]GX[YF]N;(SEQ ID NO: 10)Motif II:G[GN]GX[YF]NXX[VA]AX[EH][LF]R;(SEQ ID NO: 11)Motif III: RXXRI[QE][DEQ]S[IM]ATN;(SEQ ID NO: 12)Motif IV:S[IM]ATN[PG][EQN][FM][SDN][FL];(SEQ ID NO: 13)Motif V:P[PDK][DG]F[HFW]R[AP];(SEQ ID NO: 14)Motif VI:[TI]XXXLYPNP[TK][GV];(SEQ ID NO: 15)Motif VII:E[HG]DXSX[ST]RXD. |
