发明名称 |
METHOD OF USING NEUTRILIZED DNA (N-DNA) AS SURFACE PROBE FOR HIGH THROUGHPUT DETECTION PLATFORM |
摘要 |
A method of using Neutrilized DNA (N-DNA) as a surface probe for a high throughput detection platform is disclosed. FET and SPRi are used as high throughput detection platforms to demonstrate that the N-DNA surface probe produces good results and enhances detection sensitivity. The N-DNA modifies the charged oxygen ions (O−) on the phosphate backbone through methylation, ethylation, propylation, or alkylation, so that the backbone is not charged after this modification to increase the hybridization efficiency, sensitivity and to make the signal more clear. |
申请公布号 |
US2014235465(A1) |
申请公布日期 |
2014.08.21 |
申请号 |
US201313770987 |
申请日期 |
2013.02.19 |
申请人 |
Chen Wen-Yi;Yang Yun-Shyong;Chan Hardy Wai-Hong |
发明人 |
Chen Wen-Yi;Yang Yun-Shyong;Chan Hardy Wai-Hong |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. An Neutrilized DNA (N-DNA) surface probe used in a high throughput detection platform, wherein the N-DNA modifies the charged oxygen ions (O−) on the phosphate backbone of said N-DNA through methylation, ethylation, propylation, or alkylation, so that the backbone is not charged after this modification to increase the hybridization efficiency, sensitivity and to make the signal more clear. |
地址 |
Jhongli TW |