COMPOSITIONS AND METHODS FOR IN VITRO DIAGNOSTIC TESTS INCLUDING SULFONIC ACID

摘要

The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample.

主权项

1. A composition for colorimetric analysis comprising
an enzyme; a chromogenic reaction product; and a sulfonic acid that is a compound of Formula I:
R—SO3H wherein R is methyl, ethyl, propyl, —NH2, or mixtures thereof.