MEMBRANE-BINDING PROTEIN EXPRESSION VECTOR

摘要

A desired-protein expression vector which can be used for producing a protein composition with high efficiency and in a large quantity and for producing a protein-containing medicine in a safe manner and a method for producing a desired protein using the vector have been demanded. According to the present invention, it becomes possible to provide: a desired-protein expression vector which can express both a fusion protein (a membrane-binding protein or a fragment of the membrane binding-protein) of a desired protein and a transmembrane domain and the desired protein at the same time; a cell having the vector introduced thereinto; a method for screening for a cell having the vector introduced thereinto; a method for expressing both the desired protein and the membrane-binding protein at the same time by introducing the vector into a host cell; a cell capable of producing the desired protein, which can produce the desired protein at a high level and can be obtained by screening for a cell, into which the vector has been introduced to express the membrane-binding protein, by employing the expression amount of the membrane-binding protein as a measure; and a method for producing the desired protein using the cell.