PRODUCTION OF PARTHENOGENETIC STEM CELLS AND PATIENT-SPECIFIC HUMAN EMBRYONIC STEM CELLS USING SOMATIC CELL NUCLEAR TRANSFER

摘要

Immunocompatible pluripotent stem cells (pSCs), which include cells compatible with different patient populations or patient-specific cells, find wide application in regenerative medicine therapies. Described herein are immunocompatible pSCs generated using techniques such as parthenogenesis resulting in cells possessing desired haplotypes of reduced zygosity, antigenically compatible with multiple patient populations, or nuclear transfer allowing generation of patient-specific cells. Methods described herein related to parthenogenesis, nuclear transfer, or pSC cell line generation. Also described herein are compositions of immunocompatible pSCs and cell lines generated by the aforementioned techniques.

主权项

1. A method for generating a parthenote-derived human pluripotent stem cell line (pn-hPSC) comprising:
activating an oocyte by incubation in an activation medium; generating a parthenote by incubating the activated oocyte in a post-activation medium; forming a blastocyst by incubating the parthenote in a culture medium; and isolating inner cell mass (ICM) cells from the blastocyst, wherein the ICM cells are capable of further culturing as a pn-hPSC cell line.