Methods of Promoting Immune Tolerance

摘要

Compositions including a polynucleotide combined with a vehicle and methods of their use to induce a suppressive immune response are provided. In some embodiments the compositions induce an increase in expression of indoleamine 2,3 dioxygenase (IDO) enzyme activity in cells. The methods and compositions can be used to inhibit or reduce immune-mediated tissue destruction, to treat autoimmune diseases and inflammatory responses, to promote immune tolerance, to enhance tolerizing vaccines, to treat allergies, to treat asthma, or to enhance mucosal tolerance in subject. Methods and compositions for inducing a suppressive immune response for while minimizing undesirable side effects in the subject are also provided. An exemplary undesirable side effect is systemic release of INFγ. Exemplary compositions that can be used to induce an immune response in a subject without inducing systemic release of INFγ include compositions containing a polynucleotide lacking an immunostimulatory nucleic acid sequence complexed with a carrier.

主权项

1. A method for inhibiting immune-mediated tissue destruction in a subject comprising administering to the subject an effective amount of a particulate formulation to inhibit or reduce immune-mediated tissue destruction in the subject compared to a control, wherein the particulate formulation comprises polymeric particles combined with polynucleotides and induces indoleamine 2,3 dioxygenase expression in the subject.