Producción y utilización de bancos de genes de anticuerpos humanos (bibliotecas de anticuerpos humanos)

摘要

The gene library of human antibodies is taken from a mixture of human B-lymphocytes. - Their mRNA are converted into cDNA using oligo-dT primers. The cDNA specific to antibodies are amplified by PCR with suitable oligonucleotide primer sequences. Using an expression of the antibody-specific cDNA in a bacterial expression vector, e.g. in E. coli, a human antibody library is produced with a wide range for screening with selected antigens in vitro.