| 摘要 |
FIELD: biotechnology.SUBSTANCE: method comprises obtaining samples of highly purified DNA, fragmentation of the isolated DNA with restriction endonuclease having no recognition site in the amplified region, hydrolysis of fragmented DNA by methyl-dependent site-specific DNA-endonuclease GlaI or its isoschizomer, ligation of the universal oligonucleotide adapter to the hydrolysed DNA followed by amplification in real time using a primer and a probe complementary to the test DNA and the hybrid primer, which 3'end is complementary to at least 3 nucleotides of the 3' terminus of DNA in the test area of hydrolysis GlaI, and the remaining part is complementary to an adapter sequence, and making conclusion on the basis of a fluorescent signal on the presence of the sequence Pu(5mC)GPy.EFFECT: improved method.4 cl, 4 dwg, 5 tbl, 5 ex |
