Enzymes and methods for producing omega-3 fatty acids

摘要

The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.

主权项

1. A process for producing oil comprising eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, the process comprising the steps of (i) obtaining seed from a canola plant, the seed comprising eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid which were produced from α-linolenic acid during development of the seed and which are present in an esterified form as part of triglycerides at levels based on an efficiency of conversion of α-linolenic acid to eicosapentaenoic acid, docosapentaenoic acid or docosahexaenoic acid of at least 17.3% and an efficiency of conversion of eicosapentaenoic acid to docosapentaenoic acid of at least 60%, and (ii) extracting oil from the seed so as to thereby produce the oil.