| 摘要 |
A method for analyzing a mixture of components includes forming precursor ions from the components, alternately causing the precursor ions to pass to and to by-pass a fragmentation device, to form product ions from the precursor ions that pass to the device and to form substantially fewer product ions from precursor ions that by-pass the device, and obtaining mass spectra from product ions received from the device and from precursor ions that by-passed the device. An apparatus for analyzing a sample includes an ion source for forming precursor ions from the components of the sample, a fragmentation device for forming product ions from the precursor ions, a by-pass device disposed upstream of the fragmentation device for switchable by-pass of the fragmentation device, and a mass analyzer. |
| 主权项 |
1. An apparatus for analyzing a sample including a mixture, comprising:
an ion source; means for separating or partially separating different components of the mixture, and providing a sequential eluent of the components to the ion source over a period of time, wherein the means for separating or partially separating performs liquid chromatography, High Performance Liquid Chromatography (“HPLM”), anion exchange, anion exchange chromatography, cation exchange, cation exchange chromatography, ion pair reversed-phase chromatography, chromatography, single-dimensional electrophoresis, multidimensional electrophoresis, size exclusion, affinity or reverse-phase chromatography, Capillary Electrophoresis Chromatography (“CEC”), electrophoresis, ion mobility separation, Field Asymmetric Ion Mobility Separation (“FAIMS”) or capillary electrophoresis; a collision, fragmentation or reaction device for receiving ions from the ion source; a bypass device disposed upstream of the collision, fragmentation or reaction device wherein the bypass device is switchable to cause ions from the ion source to either pass through the collision, fragmentation or reaction device or bypass the collision, fragmentation or reaction device; a mass analyzer for obtaining mass spectra from ions received from the collision, fragmentation or reaction device, and from ions that bypass the collision, fragmentation or reaction device, and means for providing elution profiles of parent ions and pseudo-elution profiles of fragment ions and for identifying parent ions by comparing the elution profiles of the parent ions with the pseudo-elution profiles of the fragment ions. |
