Method for nucleic acids isolation

摘要

Methods for isolating nucleic acids using a solid phase having alkylene sulfonyl-containing compounds on its surface are provided. In one embodiment, the method comprises: contacting a sample containing nucleic acids with a solid phase having alkylene sulfonyl-containing compounds on its surface in a first aqueous solution to provide a loaded solid phase; washing the loaded solid phase with a second aqueous solution to provide a washed solid phase; and eluting the washed solid phase with a low ionic strength liquid to obtain the isolated nucleic acids. Kits containing a solid phase having alkylene sulfonyl-containing compounds also are provided.

主权项

1. A method for isolating nucleic acids comprising:
contacting a sample containing nucleic acids with a solid phase in a first aqueous solution having a pH from about 6 to about 9 to provide a loaded solid phase; wherein the first aqueous solution comprises one or more salts; and the surface of the solid phase comprises one or more functional groups having structural formula (I): wherein,X is NR1, S, or O;Y is —OH, —SH, —NHR2, —B(OH)2, or —B(OR3)2;R1 is hydrogen or alkyl;R2 is hydrogen, alkyl, or aryl;each R3 is independently alkyl or aryl, or alternatively, two of R3 together with the atoms to which they are bonded from a heterocyclyl; and represents the point of attachment to the solid phase; washing the loaded solid phase with a second aqueous solution having a pH from about 6 to about 9 to provide a washed solid phase; wherein the second aqueous solution comprises a detergent and one or more salts; and isolating the nucleic acids by eluting the washed solid phase with a low ionic strength liquid having a pH from about 5 to about 9 to obtain the isolated nucleic acids.