Method for extraction of fractions containing pharmacologically active ingredients with less cytotoxicity from one or more plants

摘要

A method of obtaining one or more fractions from one or more plants is disclosed. The method includes subjecting one or more parts of the one or more plants to hydro-alcoholic extraction in presence of a water-insoluble solvent to obtain one or more extracts. The method further includes subjecting the one or more extracts to one or more of de-pigmentation, de-fatting and detoxification process to obtain the one or more fractions. The one or more fractions thus obtained contain a desired pharmacologically active ingredient in concentration greater than concentrations of other pharmacologically active ingredients present in the at least one fraction. Further, the one or more fractions are less toxic as compared with corresponding one or more fractions obtained by conventional methods. In addition, the one or more fractions are therapeutically more effective in various diseases as compared to one or more fractions obtained by conventional methods.

主权项

1. A method of extracting a pharmacologically active fraction from Andrographis paniculata stems, wherein the pharmacologically active fraction includes concentrations of pharmacologically active ingredients, and wherein one of the pharmacologically active ingredients is andrographolide, comprising:
soaking stems of Andrographis paniculata in chloroform and aqueous 60% methanol for 8-12 hours; filtering the chloroform and aqueous 60% methanol to obtain a first residue and a first filtrate; settling the first filtrate to provide two immiscible layers, wherein the two immiscible layers are an aqueous methanol layer and a chloroform layer; separating the chloroform layer; concentrating the chloroform layer to obtain a first dry extract; dissolving the first dry extract in hexane; filtering the first dry extract dissolved in hexane to obtain a second residue and second filtrate; drying the second residue and storing as a first fraction; re-extracting the first fraction to obtain a second fraction; and combining the first and second fractions to provide the pharmacologically active fraction; wherein said pharmacologically active fraction treats inflammation.