Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides

摘要

Disclosed herein are compounds useful in the preparation non-natural amino acids and polypeptides that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Also disclosed herein are compound useful in the preparation of non-natural amino acid polypeptides that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such polypeptides. Typically, the modified non-natural amino acid polypeptides include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid polypeptides and modified non-natural amino acid polypeptides, including therapeutic, diagnostic, and other biotechnology uses.

主权项

1. A compound of Formula (XIX) having the structure:
[X-L]n-L1-W  (Formula XIX)wherein:
each X is a polymer having a molecular weight of about 5000 Da to about 40,000 Da; each L is —O—; L1 is absent, W is —N(R8)2, where each R8 is independently H or an amino protecting group; and n is 1 to 3; provided that L-L1-W together provide at least one hydroxylamine group or protected hydroxylamine group.