METHOD FOR THE SPATIAL ARRANGEMENT OF SAMPLE FRAGMENTS FOR AMPLIFICATION AND IMMOBILIZATION FOR FURTHER DERIVATIZATIONS

摘要

The invention relates to a method for performing a biochemical or chemical reaction for an isolated, spatially separated amplification of sample fragments during a simultaneous immobilization and spatial arrangement of the sample fragments and reaction products, the amplification products, on one or more suitable solid phases for subsequent derivatizations.

主权项

1. A method for amplification of sample fragments, comprising:
a. supplying a solid phase with a microstructure, wherein the microstructure subdivides the solid phase so that at least one, preferably several compartments are formed, and wherein capture molecules are attached to the solid phase, b. introducing at least one sample fragment into at least one compartment, c. introducing a reaction mixture for amplification in at least one compartment, d. separating and/or covering the individual compartments to obtain sealed compartments, e. amplifying each sample fragment independently in the corresponding sealed compartment to obtain an amplification products, f. wherein the amplification products generated by the amplifying in (e) are immobilized on the capture molecules.