METABOLIC TRANSISTOR IN BACTERIA

摘要

The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.

主权项

1) A method of increasing the production of a product in a bacteria, said method comprising:
a) providing a bacteria having a product pathway producing a product, wherein a competitive pathway competes with said product pathway for available carbon, said competitive pathway requiring a cofactor that is not rate limiting and not used in said product pathway; b) adding one or more diverting gene(s) under the control of a promoter to said bacteria to directly or indirectly divert said cofactor away from said competitive pathway; c) allowing expression of said diverting gene(s) and reducing levels of said cofactor, and thus reducing levels of said competitive pathway; d) thereby increasing said product pathway and said product.