CELL SORTING BY 3D FLOW AND ADHESIVE ROLLING

摘要

The present disclosure, among other things, describes cell rolling by 3D flow and adhesive rolling. In some embodiments, a device described herein includes a flow channel dimensioned to permit fluid flow therethrough; at least one 3D structure protruding from at least one surface of the flow channel; and at least one cell adhesion entity coated on at least part of at least one of the 3D structures, which adhesion entity interacts with a target cell brought into contact with the 3D structures by flow of a stream comprising the target cell through the flow channel such that the target cell's trajectory through the flow channel is diverted due to the interaction.

主权项

1. A device comprising:
a flow channel dimensioned to permit a cell stream flow therethrough; one or more 3D structures protruding from at least one surface of the flow channel, wherein the 3D structures are arranged and constructed so that at least two sets of streamlines are defined when the cell stream is flowed therethrough; at least one cell adhesion entity coated on at least part of the 3D structures, which adhesion entity interacts with a target cell in the cell stream brought into contact with the 3D structures by flow of a stream comprising the target cell through the flow channel such that the target cell's trajectory through the flow channel is diverted due to the interaction.