发明名称 |
HIGH THROUGHPUT PAIRED-END SEQUENCING OF LARGE-INSERT CLONE LIBRARIES |
摘要 |
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that improves the yield of long-distance genomic read pairs by constructing long-insert clone libraries (i.e., for example, a fosIll library or a fosCN library) and converting the long-insert clone library using inverse polymerase chain reaction amplification or shearing and recircularization of shortened fragments into a library of co-ligated clone-insert ends. The resultant jumping libraries are compatible with massively parallel sequencing techniques. The compositions and methods disclosed herein contemplate sequencing complex genomes as well as detecting chromosomal structural rearrangements. |
申请公布号 |
US2014228223(A1) |
申请公布日期 |
2014.08.14 |
申请号 |
US201113697108 |
申请日期 |
2011.05.10 |
申请人 |
Gnirke Andreas;Nicol Robert;Williams Louise;Costello Maura T.;Steelman Scott |
发明人 |
Gnirke Andreas;Nicol Robert;Williams Louise;Costello Maura T.;Steelman Scott |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
Wellesley MA US |