MASS SPECTROMETER AND MASS SPECTROMETRIC METHOD

摘要

A mass spectrometry using helium as cooling gas is performed to obtain a first mass spectrum (S1), and another mass spectrometry using argon, which is heavier than helium, as cooling gas is performed to obtain a second mass spectrum for the same sample (S2). Due to the difference between the two gases in terms of the effect of promoting dissociation of modifications, an ion peak originating from a target compound from which all the modifications have been dissociated will appear with a higher intensity on the second mass spectrum. The peak patterns of the two mass spectra are compared to locate the all-dissociated ion peak while excluding unnecessary peaks (S3). Based on that peak, the assignment of each peak is determined (S4). Such a method is effective for enhancing the accuracy of the determination of the assignment of the peaks on the mass spectra and for improving the accuracy of identification or structural analysis in the case of a mass spectrometry of a compound to which an easily dissociable modification, such as a Sialylated glycosylated peptide, is bonded.

主权项

1. A mass spectrometer including a mass analyzer for performing a mass spectrometry of ions originating from a compound to be analyzed and a detector for detecting the ions separated by the mass analyzer according to their mass-to-charge ratios, the mass spectrometer comprising:
a) a non-ion-selecting fragmentation promoter for promoting fragmentation of ions originating from a target compound without an ion-selecting operation; b) a first analysis executer for obtaining a first mass spectrum by performing a mass spectrometry of the ions originating from the target compound by using the mass analyzer, without performing a fragmentation-promoting operation by the non-ion-selecting fragmentation promoter; c) a second analysis executer for obtaining a second mass spectrum by performing a mass spectrometry of the ions originating from the target compound by using the mass analyzer after performing a fragmentation-promoting operation by the non-ion-selecting fragmentation promoter; and d) a data processor for determining an assignment of a peak originating from the target compound by comparing the first mass spectrum and the second mass spectrum.