OB-fold used as scaffold for engineering new specific binders

摘要

C:/NRPortbOCCkM 486371 .LDOC-510112013 The present invention pertains to the field of protein engineering, and provides means for obtaining stable molecu les that specificall y bind to a targ et selected amongst a lar ge variety of ligands families. In particular, the present invention provides methods for obtaining a molecule specifically binding to a target of interest, through a combinatorial mutation/selection approach with an OB-fold protein as a starting molecule. In particular, the target of interest can be of a different chemical nature form that of the native target of the OB-fold protein used as the starting molecule.