PRODUCTION OF OIL IN VEGETATIVE TISSUES

摘要

The present invention relates to compositions and methods for providing RNA interference (RNAi) vectors comprising trigalactosyldiacylglycerol (tgd) biosynthesis enzyme constructs for increasing oil content of plants. Further, the use of tgd RNAi silencing vectors in combination with co-expression of heterologous oil regulating transcription factors, such as WRINKLED1, are contemplated to overcome the reduced growth and variable levels of embryonic lethality in plants with reduced TGD protein. Additionally, plants having reduced APS1, a gene encoding a major catalytic isoform of the small subunit of AGPase (AGP(−) plants), co-expressing a heterologous oil modulating transcription factor are contemplated for use in combination with plants having reduced TGD. Oil harvested from vegetative tissues of plants comprising vectors and genes of the present invention is contemplated for use in biofuel and biodiesel products.

主权项

1. A double gene vector construct, comprising a RNAi silencing construct, wherein a first gene has a first promoter sequence in operable combination with a first fragment of a trigalactosyldiacylglycerol nucleic acid sequence in its sense orientation and a second fragment of a trigalactosyldiacylglycerol nucleic acid sequence in it's antisense orientation, and a second gene comprising a heterologous oil regulating transcription factor nucleic acid sequence.