GLUCOAMYLASE VARIANTS WITH ALTERED PROPERTIES

摘要

The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

主权项

1. A glucoamylase variant comprising two or more amino acid substitutions corresponding to position 61, 73, 417, 430, 431, 503, 511, 535, 539, or 563 of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase, wherein the glucoamylase variant has at least 80% sequence identity with SEQ ID NO: 1, 2, 3, 5, 6, 7, 8, or 9.