Nanoparticles comprising amphiphilic low molecular weight hyaluronic acid complex and a process for the preparation thereof

摘要

PURPOSE: A nanoparticle containing a low molecular weight amphiphilic hyaluronic acid composite is provided to show superior water solubility compared with an existing drug delivery system and to encapsulate high volume drugs with high efficiency. CONSTITUTION: A low molecular weight hyaluronic acid-5-beta cholanic acid composite is an amphiphilic composite in which 5-beta-cholanic acid is conjugated to hyaluronic acid with a molecular weight of 5-20 kDa. The composite forms self-aggregates in water. Nanoparticles are formed by encapsulating a hydrophobic drug in the composite. A method for preparing the nanoparticles comprises the steps of: synthesizing 5-beta cholanic acid with a cholanic acid structure using aminoethyl cholanoamide; dissolving the hyaluronic acid with a molecular weight of 5-20 kDa in an aqueous solvent to prepare a hyaluronic acid solution; dissolving aminoethyl cholanoamide in an organic solvent to prepare a cholanoamide solution; adding the cholanoamide solution to the hyaluronic acid solution and preparing a reactant solution; depositing the hyaluronic acid-5-beta cholanic acid composite from the reactant solution; and encapsulating a drug in the hyaluronic acid-5-beta cholanic acid.