发明名称 |
Methods for the production of 3-O-deactivated-4'-monophosphoryl lipid A (3D-MLA) |
摘要 |
<p>Herein is disclosed a method for producing lipopolysaccharide (LPS), comprising: (a) growing a culture of a deep rough mutant bacterial strain in a medium; (b) maintaining the culture in stationary phase for at least about 5 hr; (c) harvesting cells from the culture; and (d) extracting LPS from the cells. The method allows for the production of an LPS which can be used to produce a 3-0-deacylated monophosphoryl lipid A (3D-MLA) having at least about 20 mol% of the hexaacyl congener group. Also herein is disclosed a method of extracting lipopolysaccharide (LPS) from a culture of deep rough mutant bacterial strain cells, comprising: (a) extracting the cells with a solution consisting essentially of at least about 75 wt% of an aliphatic alcohol having from 1 to 4 carbon atoms and the balance water, thereby producing cells with reduced phospholipid content; and (b) extracting the cells with reduced phospholipid content with a solution comprising chloroform and methanol, thereby yielding a solution of LPS in chloroform and methanol (mM). This method provides LPS solutions in CM that have reduced phospholipid content and are produced by relatively simple and inexpensive process steps.</p> |
申请公布号 |
EP2465937(B1) |
申请公布日期 |
2014.08.13 |
申请号 |
EP20110176340 |
申请日期 |
2002.03.28 |
申请人 |
CORIXA CORPORATION |
发明人 |
MYERS, KENT, R.;SNYDER, D. SCOTT |
分类号 |
C12P1/04;C12P19/04;A61K31/739;A61P37/04;C08B37/00;C12N1/20;C12N1/21 |
主分类号 |
C12P1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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