INHIBITORS OF METHYLATION OF LIN28A FOR CONTROLLING DIFFERENTIATION OF PLURIPOTENT STEM CELLS, ITS SCREENING METHOD AND COMPOSITIONS OF THE SAME

摘要

The present invention relates to a composition for adjusting a pluripotent stem cell including an LIN28A methylation inhibitor and to a method for screening the LIN28A methylation inhibitor. More particularly, the present invention relates to a composition for treating cancer or adjusting a pluripotent embryonic stem cell including an inhibitor which controls the methylation of LIN28A 135th lysine which is methylated by Set7/9 and to a method for screening the inhibitor. The method of the present invention comprises the following steps: (a) making a candidate material touch a cell in which an LIN28A gene is introduced; (b) measuring the methylation level of the 135th lysine of LIN28A; and (c) selecting an inhibitor which controls the methylation of the 135th lysine of LIN28A. By means of the method of the present invention, a material which can adjust a pluripotent embryonic stem cell or a material having anticancer activities can be effectively screened. The inhibitor screened by means of the method of the present invention adjusts a pluripotent embryonic stem cell and controls the growth of a cancer cell, thereby being effective in producing an agent for treating cancer or stem cell differentiation disorders.