Arabinose isomerase expressed from <i>Corynebacterium </i>genus and tagatose manufacturing method by using it

摘要

The present invention relates to a thermophilic arabinose isomerase and a method of manufacturing tagatose using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophile Thermotoga neapolitana DSM 5068, a recombinant expression vector containing the gene, a method of preparing a food grade thermophilic arabinose isomerase from the recombinant GRAS (Generally Recognized As Safe) strain transformed with the said expression vector, and a method of preparing tagatose from galactose using the said enzyme.

主权项

1. A method for producing an arabinose isomerase comprising the steps of:
preparing a recombinant vector by inserting a gene encoding a thermophilic arabinose isomerase, which has the amino acid sequence of SEQ ID NO:3 originating from the Thermotoga neapolitana DSM 5068, into the shuttle vector pCJ-1(KCCM 10611) or pCJ-7(KCCM 10617); transfecting a Corynebacterium sp. strain with the recombinant vector; and producing the arabinose isomerase by culturing the Corynebacterium sp. strain.